ddit3 expression plasmids Search Results


human ddit3 gene orf cdna clone expression plasmid, c-ofpspark tag  (Sino Biological)
N/A
Full length Clone DNA of Human DNA-damage-inducible transcript 3 with C terminal OFPSpark / RFP tag.
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ddit3 expression plasmid  (GenScript corporation)
90
GenScript corporation ddit3 expression plasmid
( A ) Location of rs2071473 with respect to histone modifications that characterize promoters (H3K4me4 ChIP-Seq), enhancers (H3K27ac ChIP-Seq), open chromatin (DNaseI-Seq, FAIRE-Seq), as well as PGR and NR2F2 ChIP-Seq binding sites. Inset, the rs2071473-C allele is predicted to disrupt a <t>DDIT3</t> binding site. The 1kb region shown in light brown was cloned into the pGL3Basic luciferase reporter vector for functional characterization. ( B ) Luciferase assay results testing the regulatory potential of the T (pGL3Basic-rs2071473C) and C (pGL3Basic-rs2071473T) alleles in endometrial stromal fibroblasts treated with control media (ESFs) or differentiated into DSCs with cAMP/MPA for 48 hours. Data are shown as luciferase expression from the pGL3Basic-rs2071473C or pGL3Basic-rs2071473T reporter relative to renilla expression (pRL-null and empty vector (pGL3Basic) controls. +DDIT3, Relative luciferase expression from the pGL3Basic-rs2071473C or pGL3Basic-rs2071473T in ESFs and DSCs co-transfected with DDIT3. +PGR, Relative luciferase expression from the pGL3Basic-rs2071473C or pGL3Basic-rs2071473T in ESFs and DSCs co-transfected with PGR. +DDIT3 & PGR, Relative luciferase expression from the pGL3Basic-rs2071473C or pGL3Basic-rs2071473T in ESFs and DSCs co-transfected with DDIT3 and PGR. ( C ) DeepSea predicted effects (log 2 fold change) of rs2071473 on regulatory features across cell-types.
Ddit3 Expression Plasmid, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ddit3+expression+plasmids/ddit3+expression+plasmid/bio_rxiv__058388-41-0-9
Average 90 stars, based on 1 article reviews
ddit3 expression plasmid - by Bioz Stars, 2026-09
90/100 stars
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mouse ddit3 gene orf cdna clone expression plasmid, c-myc tag  (Sino Biological)
N/A
Full length Clone DNA of Mouse DNA-damage inducible transcript 3 with C terminal Myc tag.
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rat ddit3 gene lentiviral orf cdna expression plasmid, c-gfpspark tag  (Sino Biological)
N/A
Full length Clone DNA of Rattus norvegicus DNA-damage inducible transcript 3
   Buy from Supplier
human ddit3 gene orf cdna clone expression plasmid, c-his tag  (Sino Biological)
N/A
Full length Clone DNA of Human DNA-damage-inducible transcript 3 with C terminal His tag.
   Buy from Supplier
mouse ddit3 gene orf cdna clone expression plasmid, n-ofpspark tag  (Sino Biological)
N/A
Full length Clone DNA of Mouse DNA-damage inducible transcript 3 with N terminal OFPSpark / RFP tag.
   Buy from Supplier
mouse ddit3 gene orf cdna clone expression plasmid, c-his tag  (Sino Biological)
N/A
Full length Clone DNA of Mouse DNA-damage inducible transcript 3 with C terminal His tag.
   Buy from Supplier
mouse ddit3 gene orf cdna clone expression plasmid, n-ha tag  (Sino Biological)
N/A
Full length Clone DNA of Mouse DNA-damage inducible transcript 3 with N terminal HA tag.
   Buy from Supplier
human ddit3 gene orf cdna clone expression plasmid, n-his tag  (Sino Biological)
N/A
Full length Clone DNA of Human DNA-damage-inducible transcript 3 with N terminal His tag.
   Buy from Supplier
mouse ddit3 gene orf cdna clone expression plasmid, c-ofpspark tag  (Sino Biological)
N/A
Full length Clone DNA of Mouse DNA-damage inducible transcript 3 with C terminal OFPSpark / RFP tag.
   Buy from Supplier
human ddit3 gene orf cdna clone expression plasmid, n-ofpspark tag  (Sino Biological)
N/A
Full length Clone DNA of Human DNA-damage-inducible transcript 3 with N terminal OFPSpark / RFP tag.
   Buy from Supplier
mouse ddit3 gene orf cdna clone expression plasmid, c-gfpspark tag  (Sino Biological)
N/A
Full length Clone DNA of Mouse DNA-damage inducible transcript 3 with C terminal GFPSpark tag.
   Buy from Supplier

Image Search Results


( A ) Location of rs2071473 with respect to histone modifications that characterize promoters (H3K4me4 ChIP-Seq), enhancers (H3K27ac ChIP-Seq), open chromatin (DNaseI-Seq, FAIRE-Seq), as well as PGR and NR2F2 ChIP-Seq binding sites. Inset, the rs2071473-C allele is predicted to disrupt a DDIT3 binding site. The 1kb region shown in light brown was cloned into the pGL3Basic luciferase reporter vector for functional characterization. ( B ) Luciferase assay results testing the regulatory potential of the T (pGL3Basic-rs2071473C) and C (pGL3Basic-rs2071473T) alleles in endometrial stromal fibroblasts treated with control media (ESFs) or differentiated into DSCs with cAMP/MPA for 48 hours. Data are shown as luciferase expression from the pGL3Basic-rs2071473C or pGL3Basic-rs2071473T reporter relative to renilla expression (pRL-null and empty vector (pGL3Basic) controls. +DDIT3, Relative luciferase expression from the pGL3Basic-rs2071473C or pGL3Basic-rs2071473T in ESFs and DSCs co-transfected with DDIT3. +PGR, Relative luciferase expression from the pGL3Basic-rs2071473C or pGL3Basic-rs2071473T in ESFs and DSCs co-transfected with PGR. +DDIT3 & PGR, Relative luciferase expression from the pGL3Basic-rs2071473C or pGL3Basic-rs2071473T in ESFs and DSCs co-transfected with DDIT3 and PGR. ( C ) DeepSea predicted effects (log 2 fold change) of rs2071473 on regulatory features across cell-types.

Journal: bioRxiv

Article Title: An ancient fecundability-associated polymorphism switches a repressor into an enhancer of endometrial TAP2 Expression

doi: 10.1101/058388

Figure Lengend Snippet: ( A ) Location of rs2071473 with respect to histone modifications that characterize promoters (H3K4me4 ChIP-Seq), enhancers (H3K27ac ChIP-Seq), open chromatin (DNaseI-Seq, FAIRE-Seq), as well as PGR and NR2F2 ChIP-Seq binding sites. Inset, the rs2071473-C allele is predicted to disrupt a DDIT3 binding site. The 1kb region shown in light brown was cloned into the pGL3Basic luciferase reporter vector for functional characterization. ( B ) Luciferase assay results testing the regulatory potential of the T (pGL3Basic-rs2071473C) and C (pGL3Basic-rs2071473T) alleles in endometrial stromal fibroblasts treated with control media (ESFs) or differentiated into DSCs with cAMP/MPA for 48 hours. Data are shown as luciferase expression from the pGL3Basic-rs2071473C or pGL3Basic-rs2071473T reporter relative to renilla expression (pRL-null and empty vector (pGL3Basic) controls. +DDIT3, Relative luciferase expression from the pGL3Basic-rs2071473C or pGL3Basic-rs2071473T in ESFs and DSCs co-transfected with DDIT3. +PGR, Relative luciferase expression from the pGL3Basic-rs2071473C or pGL3Basic-rs2071473T in ESFs and DSCs co-transfected with PGR. +DDIT3 & PGR, Relative luciferase expression from the pGL3Basic-rs2071473C or pGL3Basic-rs2071473T in ESFs and DSCs co-transfected with DDIT3 and PGR. ( C ) DeepSea predicted effects (log 2 fold change) of rs2071473 on regulatory features across cell-types.

Article Snippet: DDIT3 and PGR expression plasmids were also obtained from Genscript.

Techniques: ChIP-sequencing, Binding Assay, Clone Assay, Luciferase, Plasmid Preparation, Functional Assay, Control, Expressing, Transfection